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Do you have to equilibrate a HILIC column more than a reversed phase column? | Trust Your Science, ep. 24

Often scientists complain about poor reproducibility from injection to injection when performing HILIC separations, something that is not typically observed with reversed-phase chromatography. Is this due to a lack of column equilibration?

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kim

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3:19
Kyungpook National University Advances Mass Spectrometry Technology Joint Laboratory
Kyungpook National University Advances Mass Spectrometry Technology Joint Laboratory
7:55
Phospholipid removal reduces matrix effects and improves column lifetime. It doesn't really matter which SPE sorbent I choose, as long as it retains my analyte, right? | Trust Your Science, S2E18
Phospholipid removal reduces matrix effects and improves column lifetime. It doesn't really matter which SPE sorbent I choose, as long as it retains my analyte, right? | Trust Your Science, S2E18
4:45
Solid-core particles: lower backpressure than fully-porous particles?
Solid-core particles: lower backpressure than fully-porous particles?
5:36
Can you retain polar acidic compounds using reversed-phase conditions? | Trust Your Science, S2E16
Can you retain polar acidic compounds using reversed-phase conditions? | Trust Your Science, S2E16
9:21
Is there a comprehensive LC-MS/MS method available to analyze over 80 compounds? | Trust Your Science, S2E14
Is there a comprehensive LC-MS/MS method available to analyze over 80 compounds? | Trust Your Science, S2E14
5:17
Solid-core columns: more efficient than fully-porous columns?
Solid-core columns: more efficient than fully-porous columns?
5:35
Doing SPE method development for acids, bases and neutrals. What about zwitterions? | Trust Your Science, S2E12
Doing SPE method development for acids, bases and neutrals. What about zwitterions? | Trust Your Science, S2E12
6:08
Is there any advantage in developing a method at basic pH? | Trust Your Science, S2E11
Is there any advantage in developing a method at basic pH? | Trust Your Science, S2E11
7:36
How can the design of an SPE device affect my analytical results? | Trust Your Science, S2E10
How can the design of an SPE device affect my analytical results? | Trust Your Science, S2E10
5:14
Are TCA Cycle metabolites impossible? | Trust Your Science, S2E9
Are TCA Cycle metabolites impossible? | Trust Your Science, S2E9
4:53
To increase recovery of sticky peptides and reduce non-specific binding in sample container, all you need to do is increase the percentage of organic in the diluent? | Trust Your Science, S2E8
To increase recovery of sticky peptides and reduce non-specific binding in sample container, all you need to do is increase the percentage of organic in the diluent? | Trust Your Science, S2E8
6:55
Do you have to equilibrate a HILIC column more than a reversed phase column? | Trust Your Science, ep. 24
Do you have to equilibrate a HILIC column more than a reversed phase column? | Trust Your Science, ep. 24
7:19
Do we need to compromise sensitivity and peak shape with TFA or formic acid? Trust Your Science, ep. 23
Do we need to compromise sensitivity and peak shape with TFA or formic acid? Trust Your Science, ep. 23
7:21
Do we need to compromise sensitivity and peak shape with TFA or formic acid? Trust Your Science, ep. 22
Do we need to compromise sensitivity and peak shape with TFA or formic acid? Trust Your Science, ep. 22
5:58
Can IEX chromatography be coupled to a MS for more detailed information? | Trust Your Science, ep. 20
Can IEX chromatography be coupled to a MS for more detailed information? | Trust Your Science, ep. 20

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